Without a doubt, a dissertation is one of the most important and hard-to-write papers. But we are able to lift this enormous burden from Doctoral Thesis Enzymology your shoulders by crafting a thoroughly researched and well-written dissertation for you Doctoral Thesis Enzymology, Supporting Point Essay, How Long Is A 1 Page Essay, An Example Of Research Proposal College essays come with Doctoral Thesis Enzymology stricter rules and guidelines as Doctoral Thesis Enzymology well as more specific formats like APA, etc. Writing college papers can also take up a lot of your time and with the many distractions and other tasks assigned to you, it can be so hard to ensure that Doctoral Thesis Enzymology the
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Abrahams, Katherine A. PhD thesis, University of Warwick. Request Changes to record. Bacterial cell survival depends on intact peptidoglycan, an extensive cell wall polymer of alternating Doctoral thesis enzymology and N-acetylmuramic acid residues, cross-linked by short peptides. Peptidoglycan biosynthesis is a viable and validated antimicrobial target; the intracellular, membrane-bound and extracellular synthetic stages provide a multitude of enzymes for interception with inhibitors.
The ultimate phase of peptidoglycan biosynthesis occurs on the extracellular face of the cytoplasmic membrane and involves the polymerisation of Lipid II the monomeric peptidoglycan precursor by the transglycosylase and transpeptidase activities of the Penicillin-Binding Proteins PBPs. The work presented in this thesis primarily focused on the biochemical characterisation of the integral membrane proteins Streptococcus pneumoniae PBP1a, PBP2b and PBP2x.
These enzymes are doctoral thesis enzymology relevant; they are essential targets of! Full-length and truncated forms of the PBPs were cloned, doctoral thesis enzymology, expressed and purified to high levels.
Two novel spectrophotometric assays were designed and developed to study the enzymology of the individual transglycosylase and transpeptidase activities of the PBPs with their natural substrate, Lipid II. Preliminary kinetic characterisations of the bifunctional PBP1a transglycosylase activity were performed and assay conditions were optimised to recreate an in vivo environment.
PBP1a active site mutants revealed that transglycosylase activity was elevated in the absence of a functional transpeptidase domain, doctoral thesis enzymology. PBP1a and PBP2x exhibited transpeptidase activity with an apparent substrate preference for glycan polymers over Lipid II. PBP2x transpeptidase activity was not detected. The recorded rates of PBP activity were insufficient to support bacterial cell integrity, highlighting a gap in the understanding of PBP requirements.
Finally, the PBPs were subjected to crystallisation trials for structural characterisations. This work provides an excellent doctoral thesis enzymology for the analysis and elucidation of PBP specificities.
Future information attained could contribute to the design of novel inhibitors, alleviating the global threat of antibiotic resistance. Request changes or add full text files to a record. Email us: wrap warwick. uk Contact Details About Us. Skip to doctoral thesis enzymology Skip to navigation. Study Research Business Alumni News About.
The Library. Login Admin. The enzymology of Streptococcus pneumoniae peptidoglycan polymerisation, doctoral thesis enzymology. Downloads per month over past year. pdf - Submitted Version Download 64Mb Preview. Date Event June
Defence of the Doctoral Thesis in Chemical Engineering with Carolina Costa FSCN research centre
, time: 35:35Theses on Enzymology | International Library for Thesis - Photon eBooks
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